Lead-dependent deposits in diverse synaptic vesicles: suggestive evidence for the presence of anionic binding sites

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · J. Neurobiol.; (United States)

DOI:https://doi.org/10.1002/neu.480180507· OSTI ID:5844757

Sulzer, D; Piscopo, I; Ungar, F; Holtzman, E

We have observed electron dense deposits dependent on incubation of aldehyde-fixed tissues with lead ions within synaptic vesicles of several types of neurons that differ in the neurotransmitters utilized and in the secretory granules of the adrenal medulla. Evidently, vesicle components that can interact with lead ions are widespread. A plausible explanation for the occurrence of the deposits is the presence of anionic binding sites within the vesicles. This would agree well with other biochemical, cytochemical, and immunocytochemical evidence, such as that indicating the presence of sulfated macromolecules in certain synaptic vesicles. Anionic binding sites could play significant roles by participating in processes such as Ca/sup 2 +/ storage, stabilization of pH gradients, or the control of osmotic phenomena.

• Research Organization: Columbia Univ., New York, NY
• OSTI ID: 5844757
• Journal Information: J. Neurobiol.; (United States), Vol. 18:5
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LEAD
RECEPTORS
UPTAKE
ANIONS
CEREBRAL CORTEX
ELECTRONS
MICE
NERVE CELLS
RATS
ANIMAL CELLS
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBRUM
CHARGED PARTICLES
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
IONS
LEPTONS
MAMMALS
MEMBRANE PROTEINS
METALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology