Detection of single rhodamine 6g molecules in levitated microdroplets

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00065a032· OSTI ID:5844675

Barnes, M D; Ng, K C; Whitten, W B; Ramsey, J M ^[1]

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1. Oak Ridge National Lab., TN (United States)

Single Rhodamine 6G (R6G) molecules in levitated glycerol microdroplets have been detected with signal-to-noise ratios of >40 using CW laser-induced fluorescence. The fluorescence signal from single R6G molecules was identified by the magnitude of the fluorescence signal and by the unique time dependence of the fluorescence count rate before photobleaching. This high sensitivity allows single molecules to be counted by use of a digital detection approach offering significantly lower detection limits than those possible with conventional detection methods. 27 refs., 6 figs.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5844675
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 65:17; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201 -- Chemical & Physicochemical Properties
400500* -- Photochemistry
ALCOHOLS
AMINES
CARBOXYLIC ACIDS
DROPLETS
DYES
FLUORESCENCE
GLYCEROL
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LASERS
LEVITATION
LUMINESCENCE
MOLECULES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PARTICLES
REAGENTS
RHODAMINES