Low density lipoprotein receptor activity in the guinea pig adrenal cortex. I. Zonal characterization and response to adrenocorticotropin

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-118-2-653· OSTI ID:5844673

Nonomura, K; Obara, T; Strott, C A

The binding of (/sup 125/I)iodo-low density lipoprotein ((/sup 125/I)iodo-LDL) to isolated membranes was determined for the outer (glomerulosa/fasciculata) and inner (reticularis) zones of the guinea pig adrenal cortex. Binding of (/sup 125/I)iodo-LDL to membranes from both zones was found to be highly specific and dependent on the presence of divalent cations, such as calcium. Saturation analysis revealed that the maximum high affinity binding capacity was similar for both zones under control conditions (approximately 400 ng/mg protein). When ACTH was administered, however, the maximum high affinity binding capacity increased in the outer zone by at least 3-fold, while there was no significant change for the inner zone. It was also noted that dexamethasone administration decreased (/sup 125/I)iodo-LDL high affinity binding activity to less than half the control level in the outer zone without altering the binding activity in the inner zone. The cholesterol content in the outer zone was 2-3 times greater than that in the inner zone. When ACTH was administered, the concentration of cholesterol decreased significantly in both zones. Thus, ACTH appeared to influence cholesterol metabolism in the outer and inner adrenocortical zones, but was able to modulate LDL receptor activity only in the outer zone.

• Research Organization: National Institute of Child Health and Human Development, Bethesda, MD
• OSTI ID: 5844673
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 2; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTH
ADRENAL GLANDS
ADRENAL HORMONES
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM
CHEMICAL BONDS
CHOLESTEROL
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DEXAMETHASONE
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENDOCRINE GLANDS
GLANDS
GLUCOCORTICOIDS
GUINEA PIGS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LIPIDS
LIPOPROTEINS
MAMMALS
MEMBRANE PROTEINS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
STEROID HORMONES
STEROIDS
STEROLS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES