Improved estimation of cholesteryl ester transfer/exchange activity in serum or plasma

Journal Article · Fri Jan 31 23:00:00 EST 1986 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5844374

Groener, J E; Pelton, R W; Kostner, G M

This simple, routine assay for measuring cholesteryl ester transfer/exchange activity in human plasma is based on the removal of interfering lipoproteins--very-low-density (VLDL) and low-density lipoproteins (LDL)--by precipitation with polyethylene glycol. High-density lipoproteins (HDL) in the samples do not affect the results. The supernate after precipitation is mixed with (/sup 14/C)cholesteryl ester-labeled LDL as donor and with HDL as the acceptor for the cholesteryl ester. After incubation for 16 h at 37 degrees C, LDL is separated from HDL by precipitation with dextran sulfate and the radioactivity measured in the supernate, which contains the HDL. The assay is applicable to samples containing as much as 10 mmol of triglycerides per liter. The within-assay CV was 2.7%, the day-to-day CV 6.8%. Results compared well with those by conventional procedures.

• Research Organization: Univ. of Graz, Austria
• OSTI ID: 5844374
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 2; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALCOHOLS
CARBON 14 COMPOUNDS
CENTRIFUGATION
CHOLESTEROL
COUNTING TECHNIQUES
ESTERS
GLYCOLS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
MATHEMATICS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
POLYETHYLENE GLYCOLS
POLYMERS
PRECIPITATION
PROTEINS
RADIOASSAY
REMOVAL
SCINTILLATION COUNTING
SEPARATION PROCESSES
STEROIDS
STEROLS
ULTRACENTRIFUGATION