Different rhodopsin monoclonal antibodies reveal different binding patterns on developing and adult rat retina
We used a battery of 10 monoclonal antibodies directed against different identified peptide sequences within the carboxyl, transmembrane loop, and amino terminal regions of rhodopsin to label retinas from early postnatal and adult rats. Intensity of label, age of initial appearance of staining, and distribution of label varied depending on the antibody. Most antibodies showed detectable labeling at postnatal day 1, and were eventually observed binding to the cell bodies and the inner and outer segments of the photoreceptors. One amino terminal and two carboxyl terminal antibodies, however, showed no detectable labeling until postnatal day 5 and were only transiently detectable in the cell body region. These patterns cannot be explained by accessibility of binding site, binding affinity, fixation artifact, or crossreactivity. The results indicate that physiological and experimental parameters can alter the apparent immunocytochemical localization of conformationally active molecules such as rhodopsin. The results also suggest that rhodopsin can undergo light-dependent conformational changes in several different compartments within rat retinal photoreceptors before the time of eye opening.
- Research Organization:
- Rockefeller Univ., New York, NY
- OSTI ID:
- 5842265
- Journal Information:
- J. Histochem. Cytochem.; (United States), Vol. 35:11
- Country of Publication:
- United States
- Language:
- English
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MONOCLONAL ANTIBODIES
BIOCHEMICAL REACTION KINETICS
RECEPTORS
RETINA
LABELLING
AMINO ACID SEQUENCE
ANTIGENS
COMPARATIVE EVALUATIONS
MICE
RADIOIMMUNOASSAY
RADIOISOTOPES
RATS
RHODOPSIN
ANIMALS
ANTIBODIES
BODY
BODY AREAS
EYES
FACE
HEAD
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
ORGANS
PIGMENTS
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
RODENTS
SENSE ORGANS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques