Inactivation of the cluster of rRNA genes in the second chromosomal pair of the Aleutian mink

Journal Article · Fri Nov 01 04:00:00 EST 1985 · Dokl. Biol. Sci. (Engl. Transl.); (United States)

OSTI ID:5841777

Grafodatskii, A S; Lushnikova, T P; Vorob'eva, N V

The authors present the results of their in situ mapping of the RNA genes in the chromosomes of Aleutian mink. The ribosomal RNA was extracted from the mink liver and analyzed electrophoretically. The 28S and 18S rRNA fractions were separated in 5-25% sucrose gradient containing 10 mM Tris-HCl, pH 7.4, 1.0 mM EDTA, and 100 mM NaCl. Centrifugation was done at 2/sup 0/C for 20 h in rotor SW-27 at 21,000 rpm. The 18S rRNA was used to synthesize cDNA in the system of RNA-dependent DNA polymerase of Escherichia coli with the exception that the template concentration was raised to 40 micrograms/ml and that of enzyme to 1080 units of activity/ml. The electrophoretic analysis in polyacrylamide gel with formamide showed that the size of /sup 3/H-cDNA was 4-10S. Specific radioactivity was 10/sup 8/ disintegrations min x microgram. Hybridization on the chromosomal preparations was done by the method described earlier. /sup 3/H-cDNA of the 18S mink RNA was applied to the preparation at the concentration of 0.05 micrograms/ml and hybridized in 3 x SSC for 24 h at 65/sup 0/C. The preparations were covered with photoemulsion type M and exposed for 4 months. The chromosomal preparations were made from the bone marrow cells by the standard method. The silver-staining of the nucleolus organizing regions (NOR) was done by the method of Bloom and Goodpasture.

• Research Organization: Institute of Cytology and Genetics, Novosibirsk, USSR
• OSTI ID: 5841777
• Journal Information: Dokl. Biol. Sci. (Engl. Transl.); (United States), Journal Name: Dokl. Biol. Sci. (Engl. Transl.); (United States) Vol. 282:1-6; ISSN DKBSA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BACTERIA
BIOSYNTHESIS
BONE MARROW CELLS
CELL CONSTITUENTS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DNA
DNA POLYMERASES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
GENES
GENETIC MAPPING
HYBRIDIZATION
INACTIVATION
INDICATORS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MAPPING
MICROORGANISMS
NITRATES
NITROGEN COMPOUNDS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANOIDS
OXYGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOENZYMATIC ASSAY
RIBOSOMES
RNA
SILVER COMPOUNDS
SILVER NITRATES
SOMATIC CELLS
SYNTHESIS
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
VERTEBRATES