Cataractogenicity and bioactivation of naphthalene derivatives in lens culture and in vivo

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Lens and Eye Toxicity Research; (USA)

OSTI ID:5837564

Lubek, B M; Kubow, S; Basu, P K; Wells, P G ^[1]

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1. Univ. of Toronto, Ontario (Canada)

The cataractogenicity of naphthalene derivatives was investigated in a lens culture system that included the lens with an intact capsule and epithelium. The in vivo cataractogenicity of naphthalene, 1000 or 2000 mg/kg ip, also was evaluated in New Zealand white and Chinchilla pigmented rabbits. A dose-related brunescence was observed in lenses incubated with 1,4-naphthoquinone in concentrations from 31.6 to 316 microM. With 316 microM naphthoquinone, lenses were totally opaque within 24 hr. No lenticular opacities were observed with 1-naphthol or 2-naphthol in incubations lasting up to 96 hr. The bioactivation of naphthalene derivatives to reactive free radical intermediates by lenses in organ culture was investigated by electron spin resonance spectrometry (ESR) using the spin trap alpha-phenyl-N-t-butylnitrone (PBN). Lenses were incubated with 316 microM naphthoquinone and 100 mM PBN for 0.25, 4 or 7 hr. A spin trapped radical product with unresolved peaks was observed with 0.25 and 7 hr incubation. No radicals were detected in the 4 hr incubation, nor in control cultures lacking either the lens, naphthoquinone or PBN. In the in vivo studies, naphthalene was cataractogenic in both albino and pigmented rabbits. The in vitro results indicate that naphthoquinone can be bioactivated by rabbit lens to a reactive free radical intermediate, which may contribute to cataractogenicity.

• OSTI ID: 5837564
• Journal Information: Lens and Eye Toxicity Research; (USA), Journal Name: Lens and Eye Toxicity Research; (USA) Vol. 6:1-2; ISSN 1042-6922; ISSN LETRE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ANIMAL TISSUES
ANIMALS
AROMATICS
ATP-ASE
BODY
BODY AREAS
CATARACTS
CONDENSED AROMATICS
CRYSTALLINE LENS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON SPIN RESONANCE
ENZYMES
EPITHELIUM
EYES
FACE
HEAD
HYDROCARBONS
HYDROLASES
IN VITRO
IN VIVO
MAGNETIC RESONANCE
MAMMALS
METABOLISM
NAPHTHALENE
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHOHYDROLASES
RABBITS
RADICALS
RESONANCE
SENSE ORGANS
SENSE ORGANS DISEASES
SENSITIVITY
TISSUES
TOXICITY
VERTEBRATES