Metabolic effects of growth factors and polycyclic aromatic hydrocarbons on cultured human placental cells of early and late gestation

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Journal of Clinical Endocrinology and Metabolism; (USA)

DOI:https://doi.org/10.1210/jcem-72-3-718· OSTI ID:5836871

Guyda, H J ^[1]

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1. McGill Univ.-Montreal Children's Hospital Research Institute, Quebec (Canada)

The metabolic effects of epidermal growth factor (EGF), insulin, insulin-like growth factor-I (IGF-I), and IGF-II were determined on human placental cells in monolayer culture obtained from early gestation (less than 20 weeks) and late gestation (38-42 weeks). Parameters studied were uptake of aminoisobutyric acid (AIB), uptake of 3-O-methylglucose and (3H)thymidine incorporation into cell protein. Since benzo(alpha)pyrene (BP) inhibits EGF binding and autophosphorylation in cultured human placental cells, particularly in early gestation, we also studied the effect of benzo(alpha)pyrene and other polycyclic aromatic hydrocarbons (PAHs) on EGF-mediated AIB uptake. The metabolic effects of EGF, insulin, and the IGFs in cultured human placental cells varied with gestational age and the growth factor studied. All three classes of growth factors stimulated AIB uptake in both early and late gestation at concentrations from 10-100 micrograms/L, well within a physiological range. However, insulin stimulation of AIB uptake was maximal at a high concentration in both early and late gestation cells, suggesting an action via type 1 IGF receptors rather than via insulin receptors. EGF stimulated 3-O-methylglucose uptake only in term placental cells. No significant stimulation of (3H)thymidine incorporation by any of the growth factors tested was seen with either early or late gestation cells. The effect of PAHs on AIB uptake by cultured placental cells was variable. BP alone stimulated AIB uptake by both very early and late gestation cells and enhanced EGF-stimulated AIB uptake. alpha-naphthoflavone alone inhibited AIB uptake at all gestational ages and inhibited EGF-stimulated AIB uptake. beta-Naphthoflavone and 3-methylcholanthrene minimally inhibited AIB uptake by early gestation cells and did not modify EGF-stimulated uptake at any gestational period.

• OSTI ID: 5836871
• Journal Information: Journal of Clinical Endocrinology and Metabolism; (USA), Journal Name: Journal of Clinical Endocrinology and Metabolism; (USA) Vol. 72:3; ISSN JCEMA; ISSN 0021-972X
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AMINO ACIDS
AMINOBUTYRIC ACID
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
BENZOPYRENE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CULTURES
CONDENSED AROMATICS
DRUGS
FETAL MEMBRANES
GLUCOSE
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HEXOSES
HORMONES
HYDROCARBONS
HYDROGEN COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
KINETICS
MEMBRANE PROTEINS
MEMBRANES
METABOLISM
MITOGENS
MONOSACCHARIDES
NEUROREGULATORS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PLACENTA
POLYCYCLIC AROMATIC HYDROCARBONS
PREGNANCY
PROTEINS
PYRIMIDINES
REACTION KINETICS
RECEPTORS
RIBOSIDES
SACCHARIDES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE