Proteolytic inactivation of a pentafunctional enzyme conjugate: coordinate protection by the first substrate
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
OSTI ID:5832229
The arom pentafunctional enzyme conjugate of Neurospora crassa was exposed to trypsin, chymotrypsin, or a protease preparation from Neurospora in the presence and absence of the first substrate, 3-deoxy-D-arabino-heptulosonate 7-phosphate. It was found that the first substrate coordinately protects all five activities from proteolytic inactivation, which indicates a conformational change induced by this compound. In addition, the data presented are consistent with the domain theory of conjugate structure. It is also argued that coordinate protection may be of physiological significance.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5832229
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 82:3; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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