Slit-scan flow cytometry of mammalian chromosomes

Journal Article · Mon Jan 01 04:00:00 EST 1979 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/27.1.374608· OSTI ID:5831736

Gray, J W; Peters, D; Merrill, J T; Martin, R; Van Dilla, M A

A flow cytometer has been constructed which measures total fluorescence and the distribution of fluorescence along isolated, stained mammalian chromosomes. In this device, chromosomes flow lengthwise at 4 m/sec through a 1-..mu..m thick laser beam. The fluorescence from each chromosome is recorded at 10 nsec intervals; the sequence of recorded values represents the distribution of fluorescence along the chromosome and is stored in the memory of a waveform recorder. The total fluorescence of each chromosome is also measured and recorded. Preliminary studies show that doublets of 1.83 ..mu..m diameter microspheres flow with their long axes parallel to the direction of flow and that the two microspheres are resolved in the slit-scan profile. Ethidium bromide stained Muntjac and Chinese hamster chromosomes have also been slit-scanned. Centromeres were resolved in many of the Nos. 1 and 2 Chinese hamster chromosomes and the Nos. 1 and X + 3 Muntjac chromosomes.

• Research Organization: Univ. of California, Livermore
• OSTI ID: 5831736
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 27:1; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CELL FLOW SYSTEMS
CHROMOSOMES
FLUORESCENCE
HAMSTERS
HISTOLOGICAL TECHNIQUES
LASERS
LUMINESCENCE
MAMMALS
RODENTS
STAINS
VERTEBRATES