Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Temperature dependence of the resonance Raman spectra of plastocyanin and azurin between cryogenic and ambient conditions. [Pseudomonas; Spinacea oleracea]

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;

Resonance Raman spectra of spinach plastocyanin and Pseudomonas aeruginosa azurin were studied as a function of temperature between 10 K and 300 K. The spectra are markedly improved both in signal/noise ratio and in resolution at low temperatures. The assignments of the resonance Raman-active vibrations are reinterpreted in view of the number and intensities of peaks observed in the low-temperature spectra. Features appear in the low-temperature spectra of azurin that may be due to copper-bound methionine. The plastocyanin spectra undergo a transition between 220 K and the melting point of water that results in dramatically narrowed peaks at lower temperature and a shift in the carbon-sulfur stretching frequency of the copper-bound cysteine, suggesting a structural change in the active site and an accompanying effect on vibrational dephasing. Considering that the structures and nonvibrational spectroscopies of these two proteins are similar, the substantial differences in the resonance Raman spectra are striking and significant. 40 references, 3 figures, 1 table.

Research Organization:
Univ. of Texas, Austin
OSTI ID:
5829204
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 81:4; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Spectrochemical studies on the blue copper protein azurin from Alcaligenes denitrificans
Journal Article · Mon Jan 12 23:00:00 EST 1987 · Biochemistry; (United States) · OSTI ID:6619982
Resonance Raman studies of blue copper proteins: effect of temperature and isotopic substitutions. Structural and thermodynamic implications
Journal Article · Mon Dec 31 23:00:00 EST 1984 · J. Am. Chem. Soc.; (United States) · OSTI ID:6712914
Resonance Raman spectroscopy of amicyanin, a blue copper protein from Paracoccus denitrificans
Journal Article · Fri Mar 04 23:00:00 EST 1988 · J. Biol. Chem.; (United States) · OSTI ID:5176105

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
CARBOXYLIC ACIDS
COPPER
CYSTEINE
DRUGS
ELEMENTS
FOOD
LASER SPECTROSCOPY
LIPOTROPIC FACTORS
METALLOPROTEINS
METALS
METHIONINE
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
PROTEINS
PSEUDOMONAS
RAMAN SPECTRA
RAMAN SPECTROSCOPY
RESOLUTION
SIGNAL-TO-NOISE RATIO
SPECTRA
SPECTROSCOPY
SPINACH
STRUCTURAL CHEMICAL ANALYSIS
TEMPERATURE DEPENDENCE
THIOLS
TRANSITION ELEMENTS
VARIATIONS
VEGETABLES