Polycyclic aromatic hydrocarbon-protein interactions. Progress report, March 1, 1979-February 28, 1980
As bovine serum albumin (BSA) undergoes acid- or base-induced conformational changes, the binding of benzo(a)pyrene (BaP) to BSA, as well as the type II uv fluorescence (380 nm) due to pyrene-like oxidation products, increases. The presence of fatty acids also enhances the binding of BaP to BSA at neutral pH, whereas the visible fluorescence of BaP is effectively quenched by fatty acids. L-Tryptophan, which is specifically bound to BSA, enhances the BaP binding and particularly the formation of pyrene-type products. Upon removal of oxygen, the production of the type I uv fluorescence (340, 357, 378 nm) probably due to BaP radicals is diminished and hydroxy-BaP derivatives are not formed. While BaP undergoes oxygen-dependent reactions with cysteine, non-carcinogenic benzo(e)pyrene does not react with cysteine. The BaP fluorescence of young collagen (from 4 to 6 weeks old rat tail tendon) is more intense than that of old collagen (from about 2 years old rat tail tendon). With increasing temperature, the former fluorescence decreases, whereas the latter increases. The denaturation temperature of both BaP-collagen and uv (365 nm)-irradiated BaP-collagen complexes does not differ from that of collagen itself.
- Research Organization:
- Boston Biomedical Research Inst., MA (USA)
- DOE Contract Number:
- EE-77-S-02-4374
- OSTI ID:
- 5826007
- Report Number(s):
- COO-4374-2; TRN: 80-002172
- Country of Publication:
- United States
- Language:
- English
Similar Records
Polycyclic aromatic hydrocarbon-protein interactions. Final report, September 1, 1977-February 28, 1982
Polycyclic aromatic hydrocarbon: protein interactions. Progress report, March 1, 1980-February 28, 1981
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
CHEMICAL REACTIONS
BENZOPYRENE
CHEMICAL REACTION YIELD
FLUORESCENCE
COLLAGEN
CYSTEINE
PROTEIN DENATURATION
TEMPERATURE DEPENDENCE
CONFIGURATION INTERACTION
OXYGEN
RADICALS
THIOLS
ULTRAVIOLET RADIATION
ULTRAVIOLET SPECTRA
AMINO ACIDS
AROMATICS
CARBOXYLIC ACIDS
CONDENSED AROMATICS
CRYOGENIC FLUIDS
ELECTROMAGNETIC RADIATION
ELEMENTS
FLUIDS
HYDROCARBONS
LUMINESCENCE
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIATIONS
SCLEROPROTEINS
SPECTRA
YIELDS
550200* - Biochemistry
560300 - Chemicals Metabolism & Toxicology
560111 - Radiation Effects on Biochemicals- In Vitro- (-1987)