Molecular cloning and chromosomal localization of one of the human glutamate receptor genes

Journal Article · Sun Sep 01 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.17.7557· OSTI ID:5823182

Puckett, C; Gomez, C M; Tung, H; Meier, T J; Hood, L ^[1]; Korenberg, J R; Chen, Xiao Ning ^[2]

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1. California Inst. of Technology, Pasadena (United States)
2. Cedar Sinai Medical Center, Los Angeles, CA (United States)

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are classified on the basis of their activation by different agonists. The agonists kainate and {alpha}-amino-3-hydroxy-5-methyl-4-isoxasolepropionic acid define a class of glutamate receptors termed kainate receptors. The authors have isolated and sequenced a human glutamate receptor (GluHI) cDNA and determined the chromosomal localization of its gene. The DNA sequence of GluHI would encode a 907-amino acid protein that has a 97% identity to one of the rodent kainate receptor subunits. Many of the changes between the predicted amino acid sequence of GluHI and the most similar rodent kainate receptor (GluRI) occur in a region of the protein encoded in rodents by an alternatively spliced exon. The extreme conservation between the human and rat kainate receptor subunits suggests that a similar gene family will encode human kainate receptors. The GluHI mRNA is widely expressed in human brain. The human gene encoding the GluHI subunit is located at 5q33. While the GluHI gene is not located near a chromosomal region associated with any human neurogenetic disorders, the homologous region on mouse chromosome 11 contains the sites of five neurologic mutations.

• OSTI ID: 5823182
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:17; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
CARBOXYLIC ACIDS
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
GENETIC MAPPING
GLUTAMIC ACID
HETEROCHROMOSOMES
HYBRIDIZATION
MAPPING
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
RECOMBINANT DNA