Analysis and comparison of nucleotide sequences encoding the genes for (NiFe) and (NiFeSe) hydrogenases from Desulfovibrio gigas and Desulfovibrio baculatus

Journal Article · Mon May 01 04:00:00 EDT 1989 · Journal of Bacteriology; (United States)

OSTI ID:5822371

Voordouw, G ^[1]; Menon, N K; LeGall, J; Choi, Euisung; Peck, Jr, H D; Przybyla, A E ^[2]

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1. Univ. of Calgary, Alberta (Canada)
2. Univ. of Georgia, Athens (United States)

The nucleotide sequences encoding the (NiFe) hydrogenase from Desulfovibrio gigas and the (NiFeSe) hydrogenase from Desulfovibrio baculatus were analyzed by the codon usage method of Staden and McLachlan. The reported reading frames were found to contain regions of low codon probability which are matched by more probable sequences in other frames. Renewed nucleotide sequencing showed the probable frames to be correct. The corrected sequences of the two small and large subunits share a significant degree of sequence homology. The small subunit, which contains 10 conserved cysteine residues, is likely to coordinate at least 2 iron-sulfur clusters, while the finding of a selenocystein codon (TGA) near the 3{prime} end of the (NiFeSe) large-subunit gene matched by a regular cysteine codon (TGC) in the (NiFe) large subunit gene indicates the presence of some of the ligands to the active-site nickel in the large subunit.

• DOE Contract Number: AC02-79ER10466
• OSTI ID: 5822371
• Journal Information: Journal of Bacteriology; (United States), Journal Name: Journal of Bacteriology; (United States) Vol. 171:5; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BACTERIA
BIOCHEMISTRY
BIOLOGICAL VARIABILITY
CHEMISTRY
COMPARATIVE EVALUATIONS
DESULFOVIBRIO
DNA SEQUENCING
ENZYMES
EVALUATION
GENE OPERONS
GENES
GENETIC VARIABILITY
HYDROGENASES
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
SULFATE-REDUCING BACTERIA