Isolation of a cDNA clone for human threonyl-tRNA synthetase

Journal Article · Mon May 01 00:00:00 EDT 1989 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.9.5.1832· OSTI ID:5821718

Kontis, K J; Arfin, S M

A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA /lambda/gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.

• Research Organization: California Univ., Irvine, CA (USA). Dept. of Chemistry
• OSTI ID: 5821718
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 9:5, Issue 5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE OPERONS
GENE AMPLIFICATION
GENE REPRESSORS
DNA SEQUENCING
LIGASES
BIOSYNTHESIS
TRANSFER RNA
DNA-CLONING
CELL CULTURES
CHO CELLS
HYBRIDIZATION
IMMUNOLOGY
MESSENGER-RNA
RNA PROCESSING
ANIMAL CELLS
CLONING
DNA HYBRIDIZATION
ENZYMES
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
550400* - Genetics
550200 - Biochemistry