Isolation of a cDNA clone for human threonyl-tRNA synthetase
Journal Article
·
· Mol. Cell. Biol.; (United States)
A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA /lambda/gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.
- Research Organization:
- California Univ., Irvine, CA (USA). Dept. of Chemistry
- OSTI ID:
- 5821718
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Journal Issue: 5 Vol. 9:5; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Thesis/Dissertation
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Thu Dec 31 23:00:00 EST 1992
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OSTI ID:7071010
Efficient procedure for transferring specific human genes into Chinese hamster cell mutants: interspecific transfer of the human genes encoding leucyl- and asparaginyl-tRNA synthetases
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OSTI ID:6596961
Related Subjects
550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOSYNTHESIS
CELL CULTURES
CHO CELLS
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENE AMPLIFICATION
GENE OPERONS
GENE REPRESSORS
HYBRIDIZATION
IMMUNOLOGY
LIGASES
MESSENGER-RNA
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
RNA
RNA PROCESSING
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSFER RNA
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOSYNTHESIS
CELL CULTURES
CHO CELLS
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENE AMPLIFICATION
GENE OPERONS
GENE REPRESSORS
HYBRIDIZATION
IMMUNOLOGY
LIGASES
MESSENGER-RNA
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
RNA
RNA PROCESSING
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSFER RNA