Factor-binding element in the human c-myc promoter involved in transcriptional regulation by transforming growth factor. beta. 1 and by the retinoblastoma gene product

Journal Article · Fri Nov 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.22.10227· OSTI ID:5821556

Pietenpol, J A; Stein, R W; Moses, H L ^[1]; Muenger, K; Howley, P M ^[2]

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1. Vanderbilt Univ., Nashville, TN (United States)
2. National Cancer Inst., Bethesda, MD (United States)

Previous studies have shown that transforming growth factor {beta}1 (TGF-{beta}1) inhibition of keratinocyte proliferation involves suppression of c-myc transcription, and indirect evidence has suggested that the retinoblastoma gene product (pRB) may be involved in this process. In this study, transient expression of pRB in skin keratinocytes was shown to repress transcription of the human c-myc promoter region was required for regulation by both TGF-{beta}1 and pRB. These sequences, termed the TGF-{beta} control element (TCE), lie between positions {minus}86 and {minus}63 relative to the P1 transcription start site. Oligonucleotides containing the TCE bound to several nuclear factors in mobility-shift assays using extracts from cells with or without normal pRB. Binding of some factors was inhibited by TGF-{beta}1 treatment of TGF-{beta}-sensitive but not TGF-{beta}-insensitive cells. These data indicate that pRB can suppress c-myc transcription and growth inhibition.

• OSTI ID: 5821556
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:22; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
BIOCHEMISTRY
ONCOGENES
GENE REGULATION
TUMOR CELLS
CELL PROLIFERATION
ELECTROPHORESIS
GENE REPRESSORS
MAN
OLIGONUCLEOTIDES
SKIN
TRANSCRIPTION
ANIMAL CELLS
ANIMALS
BODY
CHEMISTRY
GENES
MAMMALS
MITOGENS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques