Entry of (/sup 3/H)water and (1,2-/sup 14/C)polyethylene glycol 4000 into normal and cystic fibrosis salivary mucus
This investigation was performed to compare the entry of (/sup 3/H)water and (/sup 14/C)PEG into samples of salivary mucus from patients with CF and normal subjects. A solution containing both radioisotopes was added to samples of salivary mucus and mixed, and entry of each into mucus was determined by their dilution in the supernatant at 1, 2, 4, and 24 hr. (/sup 3/H)water rapidly entered and equilibrated with the water content of both normal and CF mucus. (/sup 14/C)PEG entered mucus more slowly than (/sup 3/H)water and by 24 hr had entered at a ratio of only 0.794 +/- 0.108 and 0.766 +/- 0.039 of the water space accessible to (/sup 3/H)water in normal and CF mucus, respectively. The mechanisms responsible for the partial exclusion of the large polyethylene glycol molecule were not explored but may reflect steric restriction of access to a portion of mucus water. Thus water entry and solute exclusion, both determinants of permeability, were unaltered in CF mucus, which fact provides evidence against the presence of a generalized defect of mucus permeability in this disease. The techniques developed for this study may be adaptable to the study of the permeability properties of mucus from other sources and other gels.
- Research Organization:
- Department of Medicine, University of Saskatchewan, Saskatoon, Canada
- OSTI ID:
- 5821399
- Journal Information:
- J. Lab. Clin. Med.; (United States), Vol. 101:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Antisense oligodeoxynucleotide to the cystic fibrosis gene inhibits anion transport in normal cultured sweat duct cells
Effect of chest physiotherapy on the removal of mucus in patients with cystic fibrosis
Related Subjects
BODY FLUIDS
PERMEABILITY
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
TRITIUM OXIDES
CHILDREN
HEREDITARY DISEASES
PATIENTS
POLYETHYLENE GLYCOLS
SALIVARY GLANDS
SECRETION
AGE GROUPS
ALCOHOLS
BIOLOGICAL MATERIALS
BODY
CHALCOGENIDES
DISEASES
GLANDS
GLYCOLS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
OXIDES
OXYGEN COMPOUNDS
POLYMERS
TRITIUM COMPOUNDS
550601* - Medicine- Unsealed Radionuclides in Diagnostics