Biokinetic constants of a mixed microbial culture with model diesel fuel

Journal Article · · Hazardous Waste and Hazardous Materials; (USA)

DOI:https://doi.org/10.1089/hwm.1989.6.145· OSTI ID:5818865

Goldsmith, Jr, C D ^[1]; Balderson, R K ^[2]

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1. Sybron Chemicals, Inc., Salem, VA (USA)
2. Baylor Univ., Waco, TX (USA)

The objective of this investigation was to determine the aerobic biokinetic constants for a mixed culture utilizing diesel fuel as a substrate. Bacterial strains capable of degrading diesel fuel were obtained for this study by enrichment culturing. These were identified as Pseudomonas putida, Pseudomonas alcaligenes, Pseudomonas aeruginosa and Arthrobacter crystallopoietes. Several substrate concentrations were monitored for utilization using 500 mL gas sampling bulbs containing minimal medium. These gas tight, batch vessels were used to determine biodegradation in a closed, aqueous system. The fuel was readily degraded with a maximum utilization rate of 0.75 hours-1. The maximum growth rate was 0.55 hours-1. The half saturation constant, Ks, was determined to be 81.22 mg/L. The cell yield was found to be 0.73 mg/L biomass as suspended solids produced per mg/L substrate as COD. Growth of each of four bacterial isolates on BTX, n-dodecane, 1-tridecene, naphthalene and anthracene in minimal medium was investigated. The least substrate specificity was shown by Arthrobacter crystallopoietes and Pseudomonas putida. Napthalene was the most readily degraded by all isolates.

• OSTI ID: 5818865
• Journal Information: Hazardous Waste and Hazardous Materials; (USA), Journal Name: Hazardous Waste and Hazardous Materials; (USA) Vol. 6:2; ISSN 0882-5696; ISSN HWHME
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020900* -- Petroleum-- Environmental Aspects
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ANTHRACENE
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL VARIABILITY
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
DIESEL FUELS
DODECANE
GENETIC VARIABILITY
GROWTH
HYDROCARBONS
MICROORGANISMS
NAPHTHALENE
OIL SPILLS
ORGANIC COMPOUNDS
PETROLEUM PRODUCTS
PSEUDOMONAS