Abscisic acid accumulation in spinach leaf slices in the presence of penetrating and nonpenetrating solutes

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.77.1.25· OSTI ID:5818104

Creelman, R A; Zeevaart, J A.D.

Abscisic acid (ABA) accumulated in detached, wilted leaves of spinach (Spinacia oleracea L. cv Savoy Hybrid 612) and reached a maximum level within 3 to 4 hours. The increase in ABA over that found in detached turgid leaves was approximately 10-fold. The effects of water stress could be mimicked by the use of thin slices of spinach leaves incubated in the presence of 0.6 molar mannitol, a compound which causes plasmolysis (loss of turgor). When spinach leaf slices were incubated with ethylene glycol, a compound which rapidly penetrates the cell membrane causing a decrease in the osmotic potential of the tissue and only transient loss of turgor, no ABA accumulated. Spinach leaf slices incubated in both ethylene glycol and mannitol had ABA levels similar to those found when slices were incubated with mannitol alone. Increases similar to those found with mannitol also occurred when Aquacide III, a highly purified form of polyethylene glycol, was used. When spinach leaf slices were incubated with solutes which are supposed to disturb membrane integrity no increase in ABA was observed. These data indicate that, with respect to the accumulation of ABA, mannitol caused a physical stress rather than a chemical stress.

• Research Organization: Michigan State Univ., East Lansing
• DOE Contract Number: AC02-76ER01338
• OSTI ID: 5818104
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 77:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ABSCISIC ACID
ALCOHOLS
AUXINS
CARBOXYLIC ACIDS
DIFFUSION
FOOD
GLYCOLS
HYDROXY COMPOUNDS
LEAVES
MEMBRANE TRANSPORT
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OSMOSIS
PLANT GROWTH REGULATORS
PLANTS
SPINACH
UPTAKE
VEGETABLES