Casein genes of Bos taurus. II. Isolation and characterization of the /beta/-casein gene

Journal Article · Tue Nov 01 04:00:00 EST 1988 · Sov. Genet. (Engl. Transl.); (United States)

OSTI ID:5814957

Gorodetskii, S I; Tkach, T M; Kapelinskaya, T V

The expression of the casein genes in the cells of the mammary gland is regulated by peptide and steroid hormones. In order to study the controlling mechanisms we have isolated and characterized the /beta/-casein gene. The gene is 8.6 kb long and exceeds by a factor of 7.8 the length of the corresponding mRNA which is encoded by nine exons. The genomic clones incorporate in addition 8.5 kb and 4.5 kb of the 5/prime/- and 3/prime/-flanking regions. We have determined the sequence of the 5- and 3-terminals of the gene and have performed a comparative analysis of the corresponding regions of the rat /beta/-casein gene. Furthermore we have identified the conversed sequences identical or homologous to the potential sections of binding to the nuclear factor CTF/NF-1 by glucocorticoid and progesterone receptors. The regulatory region of the bovine casein gene contains two variants of the TATA signal, flanking the duplication section in the promoter region.

• Research Organization: N.I. Vavilov Institute of General Genetics, Moscow (USSR)
• OSTI ID: 5814957
• Journal Information: Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 24:5; ISSN SOGEB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CASEIN
CATTLE
CELL CONSTITUENTS
CELL DIFFERENTIATION
CHEMICAL REACTIONS
CLONING
COMPARATIVE EVALUATIONS
COWS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DNA
DNA HYBRIDIZATION
DNA POLYMERASES
DNA SEQUENCING
DNA-CLONING
DOMESTIC ANIMALS
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYMES
ESTERASES
FOOD
GENE REGULATION
GENES
GENOME MUTATIONS
GLANDS
HORMONES
HYBRIDIZATION
HYDROLASES
HYDROLYSIS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MAMMALS
MAMMARY GLANDS
MATERIALS
MEMBRANE PROTEINS
MICROORGANISMS
MILK
MUTATIONS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PARASITES
PEPTIDE HORMONES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PLASMIDS
POLYMERASES
PROTEINS
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RATS
RECEPTORS
RECOMBINANT DNA
RNA-ASE
RODENTS
RUMINANTS
SOLVOLYSIS
STEROID HORMONES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
VIRUSES