Dynamics of proton diffusion within the hydration layer of phospholipid membrane

Journal Article · Tue Apr 04 04:00:00 EDT 1989 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00433a029· OSTI ID:5814654

Gutman, M; Nachliel, E; Moshiach, S

The diffusion of protons at the immediate vicinity of (less than 10 A from) a phospholipid membrane is studied by the application of the laser-induced proton pulse. A light-sensitive proton emitter (8-hydroxypyrene-1,3,6-trisulfonate) was trapped exclusively in the hydration layers of multilamellar vesicles made of egg phosphatidylcholine, and the protons were dissociated by a synchronizing laser pulse. The recombination of the proton with pyranin anion was monitored by time-resolved spectroscopy and analyzed by a diffusion-controlled formalism. The measured diffusion coefficient is only slightly smaller than the diffusion coefficient of proton in bulk water. Modulating the width of the hydration layer by external pressure had a direct effect on the diffusibility of the proton: the narrower the hydration layer, the slower is the diffusion of protons.

• Research Organization: Tel Aviv Univ., Ramat Aviv (Israel)
• OSTI ID: 5814654
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 28:7; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BARYONS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
DIFFUSION
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ESTERS
FERMIONS
FLUORESCENCE
HADRONS
LASER RADIATION
LASERS
LIPIDS
LIPOSOMES
LUMINESCENCE
MEMBRANE TRANSPORT
MEMBRANES
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
PHOSPHOLIPIDS
PROTONS
RADIATIONS