Cadmium-binding proteins in midgut gland of freshwater crayfish Procambarus clarkii

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Bull. Environ. Contam. Toxicol.; (United States)

DOI:https://doi.org/10.1007/BF01699406· OSTI ID:5813860

Del Ramo, J; Pastor, A; Torreblanca, A; Medina, J; Diza-Mayans, J

Metallothioneins, metal binding proteins, were originally isolated and characterized by Margoshes and Vallee. These proteins have a high affinity for various heavy metals, particularly cadmium and mercury and have extensively been studied in mammals. Metal binding proteins have been observed in a variety of marine invertebrates; however, there is very little information available on metal binding proteins in freshwater invertebrates, and particularly in freshwater crustaceans. Cadmium is an ubiquitous non essential element which possesses high toxicity to aquatic organisms. Cadmium binding proteins observed in invertebrates have similar characteristics to mammalian metallothioneins. In 1978, the American red crayfish appeared in Albufera Lake and the surrounding rice fields (Valencia, Spain). Albufera Lake and the surrounding rice fields waters are subjected to very heavy loads of sewage and toxic industrial residues (including heavy metals) from the many urban and wastewaters in this area. In previous reports the authors studied the toxicity and accumulation of cadmium on Procambarus clarkii of Albufera Lake. This crayfish shows a high resistance to cadmium and a great accumulation rate of this metal in several tissues, including midgut gland. Since Procambarus clarkii shows a high resistance to cadmium, the presence of cadmium binding proteins (Cd-BP) in midgut gland of these crayfish would be expected. This report describes results on the characterization of Cd-BPs obtained from cadmium exposed crayfish Procambarus clarkii, demonstrating their presence in this freshwater crayfish.

• Research Organization: Univ. of Valencia (Spain)
• OSTI ID: 5813860
• Journal Information: Bull. Environ. Contam. Toxicol.; (United States), Vol. 42:2
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CADMIUM COMPOUNDS
BIOLOGICAL ACCUMULATION
DECAPODS
SENSITIVITY
METALLOPROTEINS
MOLECULAR STRUCTURE
CHEMICAL WASTES
LAKES
SEWAGE
SPAIN
WASTE WATER
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
CRUSTACEANS
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EUROPE
HYDROGEN COMPOUNDS
INVERTEBRATES
LIQUID WASTES
ORGANIC COMPOUNDS
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PROTEINS
SURFACE WATERS
WASTES
WATER
WESTERN EUROPE
560300* - Chemicals Metabolism & Toxicology