In vitro biosynthesis and membrane association of photosynthetic reaction center subunits for Rhodopseudomonas sphaeroides

Journal Article · Sat Mar 01 04:00:00 EST 1986 · J. Bacteriol.; (United States)

OSTI ID:5810392

Hoger, J H; Chory, J; Kaplan, S

The reaction center of Rhodopseudomonas sphaeroides is an integral membrane protein complex responsible for primary photochemical charge separation in photosynthesis. The authors report the synthesis of two of the three subunits of the photosynthetic reaction center using a DNA-directed in vitro transcription-translation system prepared from R. sphaeroides. The in vitro-synthesized polypeptides, as resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, had apparent M/sub r/s of 24,000 and 21,000 and were shown to be synthesized in equimolar amounts. This corresponds precisely to the in vivo reaction center subunits M and L, respectively. The invitro-synthesized polypeptides were immunoprecipitated with antibody prepared against whole native reaction centers. In addition, the identity of the in vitro-synthesized polypeptides as L and M was verified by comparing the protease digestion products of in vivo- with in vitro-synthesized reaction center subunits. Both of the in vitro-synthesized polypeptides were also found to partition with the particulate material in the transcription-translation system and to associate with added membranes.

• Research Organization: Univ. of Illinois, Urbana-Champaign
• OSTI ID: 5810392
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 165:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOSYNTHESIS
CHEMICAL REACTIONS
ELECTROPHORESIS
IN VITRO
MEMBRANES
MICROORGANISMS
ORGANIC COMPOUNDS
PEPTIDES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHOTOSYNTHETIC MEMBRANES
PHOTOSYNTHETIC REACTION CENTERS
POLYPEPTIDES
PROTEINS
RHODOPSEUDOMONAS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS