Molecular structure of the human argininosuccinate synthetase gene: Occurrence of alternative mRNA splicing
The human genome contains one expressed argininosuccinate synthetase gene and ca. 14 pseudogenes that are dispersed to at least 11 human chromosomes. Eleven clones isolated from a human genomic DNA library were characterized extensively by restriction mapping, Southern blotting, and nucleotide sequencing. These 11 clones represent the entire expressed argininosuccinate synthetase gene that spans 63 kilobases and contains at least 13 exons. The expressed gene codes for two mRNAs that differ in their 5' untranslated sequences and arise by alternative splicing involving the inclusion or deletion of an entire exon. In normal human liver and cultured fibroblasts, the predominant mature argininosuccinate synthetase mRNA lacks sequences encoded by exon 2 in the expressed gene. In contrast, the predominant argininosuccinate synthetase mRNA in baboon liver contains exon 2 sequences. A transformed canavanine-resistant human cell line in which argininosuccinate synthetase activity is 180-fold higher than that in wild-type cells contains abundant amounts of both forms of the argininosuccinate synthetase mRNA. The mRNA lacking exon 2 sequences is the more abundant mRNA species in the canavanine-resistant cells. These observations show that splicing of the argininosuccinate synthetase mRNA is species specific in primates and varies among different human cell types.
- Research Organization:
- Dept. of Biological Chemistry, Univ. of Michigan Medical School, Ann Arbor, MI 48109
- OSTI ID:
- 5810347
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 4:10
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mutations in argininosuccinate synthetase mRNA of Japanese patients, causing classical citrullinemia
Molecular analysis of human argininosuccinate lyase: Mutant characterization and alternative splicing of the coding region
Related Subjects
LIGASES
GENES
GENETIC MAPPING
MOLECULAR STRUCTURE
RNA PROCESSING
SPECIES DIVERSITY
ARGINASE
ARGININE
BABOONS
CELL CULTURES
CHROMOSOME LOSSES
CHROMOSOMES
CONCANAVALIN
DNA SEQUENCING
ELECTROPHORESIS
GENE REGULATION
GENE REPRESSORS
GENETIC EFFECTS
LIVER
MAN
MESSENGER-RNA
MOLECULAR BIOLOGY
NUCLEOTIDES
SUCCINIC ACID
AGGLUTININS
AMIDASES
AMINO ACIDS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
DICARBOXYLIC ACIDS
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HEMAGGLUTININS
HYDROLASES
LECTINS
LOSSES
MAMMALS
MAPPING
MONKEYS
NON-PEPTIDE C-N HYDROLASES
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry