Molecular structure of the human argininosuccinate synthetase gene: Occurrence of alternative mRNA splicing

Journal Article · Mon Oct 01 04:00:00 EDT 1984 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.4.10.1978· OSTI ID:5810347

Freytag, S O; Beaudet, A L; Bock, H G.O.; O'Brien, W E

The human genome contains one expressed argininosuccinate synthetase gene and ca. 14 pseudogenes that are dispersed to at least 11 human chromosomes. Eleven clones isolated from a human genomic DNA library were characterized extensively by restriction mapping, Southern blotting, and nucleotide sequencing. These 11 clones represent the entire expressed argininosuccinate synthetase gene that spans 63 kilobases and contains at least 13 exons. The expressed gene codes for two mRNAs that differ in their 5' untranslated sequences and arise by alternative splicing involving the inclusion or deletion of an entire exon. In normal human liver and cultured fibroblasts, the predominant mature argininosuccinate synthetase mRNA lacks sequences encoded by exon 2 in the expressed gene. In contrast, the predominant argininosuccinate synthetase mRNA in baboon liver contains exon 2 sequences. A transformed canavanine-resistant human cell line in which argininosuccinate synthetase activity is 180-fold higher than that in wild-type cells contains abundant amounts of both forms of the argininosuccinate synthetase mRNA. The mRNA lacking exon 2 sequences is the more abundant mRNA species in the canavanine-resistant cells. These observations show that splicing of the argininosuccinate synthetase mRNA is species specific in primates and varies among different human cell types.

• Research Organization: Dept. of Biological Chemistry, Univ. of Michigan Medical School, Ann Arbor, MI 48109
• OSTI ID: 5810347
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:10; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
AMIDASES
AMINO ACIDS
ANIMALS
ANTIBODIES
ARGINASE
ARGININE
BABOONS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CELL CULTURES
CHROMOSOME LOSSES
CHROMOSOMES
CONCANAVALIN
DICARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DNA SEQUENCING
ELECTROPHORESIS
ENZYMES
GENE REGULATION
GENE REPRESSORS
GENES
GENETIC EFFECTS
GENETIC MAPPING
GLANDS
HEMAGGLUTININS
HYDROLASES
LECTINS
LIGASES
LIVER
LOSSES
MAMMALS
MAN
MAPPING
MESSENGER-RNA
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
MONKEYS
NON-PEPTIDE C-N HYDROLASES
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RNA
RNA PROCESSING
SPECIES DIVERSITY
STRUCTURAL CHEMICAL ANALYSIS
SUCCINIC ACID
VERTEBRATES