Characteristics of alveolar macrophages following the deposition of a low burden of iron oxide in the lung

Journal Article · Tue Jan 01 04:00:00 EST 1985 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398509530793· OSTI ID:5810182

Lehnert, B E; Morrow, P E

Rats were exposed to aerosols of iron-59 oxide (mass median aerodynamic diameter, MMAD = 1.6 ..mu..m, sigma/sub g/ = 3.0) at a nominal concentration of 20 mg/m/sup 3/ for 2 h to determine how a low lung burden (approx.30 ..mu..g) of innocuous particles affects the size of the alveolar macrophage (AM) pool. The functional status of the AM was also determined as assessed in vitro by their ability to exclude Trypan blue, adhere to plastic substrate, and bind phagocytize sheep erythrocytes opsonized with immunoglobulin G (SRBC-IgG). Iron oxide deposition did not bring about significant changes in cell types or numbers of AM lavaged, AM viabilities, or the plastic substrate adherence characteristics of the AM. As of 1 d post exposure, however, the ability of AM to phagocytize SRBC-IgG increased. Phagocytosis was maximally enhanced 3-7d post exposure and returned to control levels by 20 d after exposure. The increase in phagocytic activity correlated with an increase in AM avidities for SRBC-IgG. The kinetics of subsidence of the phagocytic response did not parallel the alveolar clearance rate of the deposited particles (t/sub 1/2/ (biol) 53 d). These studies show the deposition of a low lung burden of a noncytotoxic dust can transiently enhance FC..gamma..-receptor-mediated particle binding and phagocytosis by AM.

• Research Organization: Univ. of Rochester, NY
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 5810182
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 16:6; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
AEROSOLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CLEARANCE
COLLOIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISPERSIONS
ELEMENTS
EVEN-ODD NUCLEI
EXCRETION
INTERMEDIATE MASS NUCLEI
IRON
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LAVAGE
LUNG CLEARANCE
LUNGS
MACROPHAGES
MAMMALS
METALS
NUCLEI
ORGANS
PARTICLE SIZE
PHAGOCYTES
RADIOISOTOPES
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RETENTION
RODENTS
SIZE
SOLS
SOMATIC CELLS
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES
VIABILITY