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Assignment of the chloramphenicol resistance gene to mitochondrial deoxyribonucleic acid and analysis of its expression in cultured human cells

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.1.8.697· OSTI ID:5809732

The mitochondrial deoxyribonucleic acids (mtDNA's) from human HeLa and HT1080 cells differed in their restriction endonuclease cleavage patterns for HaeII, HaeIII, and HhaI. HaeII digestion yielded a 9-kilobase fragment in Ht1080, which was replaced by 4.5-, 2.4-, and 2.1-kilobase fragments in HeLa. HaeIII and HhaI yielded distinctive 1.35- and 0.68-kilobase HeLa fragments. These restriction endonuclease polymorphisms were used as mtDNA markers in HeLa-HT1080 cybrid and hybrid crosses involving the cytoplasmic chloramphenicol resistance mutation was used. Three cybrids and four hybrids (four expressing HeLa and three expressing HT1080 chloramphenicol resistance) contained 2- to 10-fold excesses of the mtDNA of the chloramphenicol-resistant parent. One cybrid, which was permitted to segregate chloramphenicol resistance and was then rechallenged with chloramphenicol, had approximately equal proportions of the two mtDNA's. Only one hybrid was discordant. These results indicated that chloramphenicol resistance is encoded in mtDNA and that expression of chloramphenicol resistance is related to the ratio of chloramphenicol-resistant and -sensitive genomes within cells.

Research Organization:
Dept. of Genetics, School of Medicine, Stanford Univ., Stanford, CA 94305
OSTI ID:
5809732
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 1:8; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550300 -- Cytology
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL MARKERS
CELL CONSTITUENTS
CELL CULTURES
CHEMICAL REACTION KINETICS
CHLORAMPHENICOL
DNA
DNA-ASE
DRUGS
ENDONUCLEASES
ENZYMES
ESTERASES
GENES
GENOME MUTATIONS
HELA CELLS
HYBRIDIZATION
HYDROLASES
KINETICS
MAMMALS
MAN
MESSENGER-RNA
MITOCHONDRIA
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHODIESTERASES
PRIMATES
REACTION KINETICS
RNA
SENSITIVITY
STRAND BREAKS
VERTEBRATES