Identification and cloning of genes involved in specific desulfurization dibenzothiophene by Rhodococcus sp. strain IGTS8

Journal Article · Wed Sep 01 04:00:00 EDT 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:5809499

Denome, S A; Young, K D; Olson, E S ^[1]

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1. Univ. of North Dakota School of Medicine, Energy and Environmental Research Center, Grand Forks, ND (United States)

The presence of sulfur in coal and petroleum contributes to corrosion of production and refining equipment and burning these high-sulfur products emits sulfur oxides to the atmosphere. Microorganisms that can enzymatically release organically bound sulfur from organic components of coal or petroleum or from dibenzothiophene (DBT) could reduce the sulfur content of high sulfur fuels without depleting their British thermal unit value. Two major pathways for microbial metabolism of DBT have been proposed. Some of the genes for the DPT degradative pathway have been isolated and characterized. However, no genes for the desulfurization pathway have been identified. This paper reports the isolation from Rhodoccus sp. strain IGTS8 pf a set of genes that confer a specific desulfurization phenotype to mutants and to a related organism, R. fascians D188-5, that is normally unable to desulfurize DBT. 38 refs., 2 figs., 1 tab.

• DOE Contract Number: AC22-89PC89901
• OSTI ID: 5809499
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 59:9; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CARBONACEOUS MATERIALS
CHEMICAL REACTIONS
CLONING
COAL
CORROSION
DECOMPOSITION
DESULFURIZATION
ENERGY SOURCES
FOSSIL FUELS
FUELS
GENES
HETEROCYCLIC COMPOUNDS
MATERIALS
METABOLISM
MICROORGANISMS
MUTANTS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PETROLEUM
PHENOTYPE
RHODOCOCCUS
SULFUR CONTENT
SULFUR-OXIDIZING BACTERIA
THIONAPHTHENES