Chromosome-specific cDNAs/STSs
This project has three main goals: (1) to construct high-quality normalized cDNA libraries from human tissues, (2) to develop methods for isolation of chromosome-specific cDNAs, (3) to contribute sequence information to the expanding cDNA/EST database. Our short term goal has been to construct a very high quality cDNA library from a 3 month post-natal total human brain. Several libraries were constructed and carefully characterized by DNA sequencing and restriction analysis while protocols were being streamlined. We have now generated a library which has the following main features: (1) very high complexity (10{sup 7} recombinants), (b) short poly (A) tails; (c) long size inserts (1 kb average); (d) undetectable co-cloning events; (e) background of non-recombinants. The next step will be to generate and test a normalized version of this brain cDNA library. Subsequently, single-stranded versions of the normalized library will be utilized for isolation of chromosome 13-specific cDNAs.
- Research Organization:
- Columbia Univ., New York, NY (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-91ER61233
- OSTI ID:
- 5809398
- Report Number(s):
- DOE/ER/61233-1; CONF-9109321-1; CONF-9110302-1; ON: DE92004022
- Resource Relation:
- Conference: 3. genome sequencing conference; International symposium on identification of transcribed sequences, Hilton Head, SC (United States); Washington, DC (United States), 22-25 Sep 1991; 4-5 Oct 1991
- Country of Publication:
- United States
- Language:
- English
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Chromosome-specific cDNAs/STSs
Chromosome-specific cDNAs/STSs. Progress report, March 1, 1992--February 28, 1993