Enhanced replication of UV-damaged Simian virus 40 DNA in carcinogen-treated mammalian cells

Thesis/Dissertation · Fri Dec 31 23:00:00 EST 1982

OSTI ID:5804673

Maga, J A

The replication of UV-damaged Simian virus 40 (SV40) in carcinogen-treated monkey cells has been studied to elucidate the mechanism of carcinogen-enhanced reactivation. Carcinogen enhanced reactivation is the observed increase in UV-irradiated virus survival in host cells treated with low doses of carcinogen compared to UV-irradiated virus survival in untreated hosts. Carcinogen treatment of monkey kidney cells with either N-acetoxy-2-acetylaminofluorene (AAAF) or UV radiation leads to an enhanced capacity to replicate UV-damaged virus during the first round of infection. To further define the mechanism leading to enhanced replication, a detailed biochemical analysis of replication intermediates in carcinogen-treated cells was performed. Several conclusions can be drawn. First enhanced replication can be observed in the first four rounds of replication after UV irradiation of viral templates. The second major finding is that the relaxed circular intermediate model proposed for the replication of UV-damaged templates in untreated cells appears valid for replication of UV-damaged templates in carcinogen-treated cells. Possible mechanisms and the supporting evidence are discussed and future experiments outlined.

• Research Organization: California Univ., Los Angeles (USA)
• OSTI ID: 5804673
• Country of Publication: United States
• Language: English

Related Subjects

560112 -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CONDENSED AROMATICS
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
EXTREME ULTRAVIOLET RADIATION
HYDROCARBONS
KIDNEYS
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
RADIATION EFFECTS
RADIATIONS
SIMIAN VIRUS
ULTRAVIOLET RADIATION
VIRUSES