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New reagent system for spectrophotometric determination of benzidine

Journal Article · · Am. Ind. Hyg. Assoc. J.; (United States)
The present method is based on the diazotization of benzidine and its subsequent coupling with 8-hydroxyquinoline. The red colored dye so formed in alkaline medium is extracted into 3-methyl-1-butanol. The Beer's law is obeyed in the range of 0.03 to 0.30 ..mu..g/mL. The molar absorptivity and Sandell's sensitivity are 6.4 x 10/sup 4/ L/mol/cm and 0.0026 ..mu..g/cm/sup 2/ respectively. Other reaction conditions and analytical parameters were also studied. The present method has been tested for the determination of benzidine in urine.
Research Organization:
Ravishankar Univ., Raipur, India
OSTI ID:
5804206
Journal Information:
Am. Ind. Hyg. Assoc. J.; (United States), Journal Name: Am. Ind. Hyg. Assoc. J.; (United States) Vol. 46:9; ISSN AIHAA
Country of Publication:
United States
Language:
English