Selectively eliminated blood monocytes and splenic suppressor macrophages in mice depleted of bone marrow by strontium 89

Technical Report · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5802744

Dempsey, W L; Morahan, P S

The contribution of specific activity to the effects of the bone-seeking isotope, /sup 89/Sr on radiosensitive components of mononuclear phagocyte populations was investigated in mice. CBA/J mice received a fixed dose of 2 microcuries/g body weight of /sup 89/Sr with three different specific activities, 6 Ci, 100 microcuries, and 20 /sup 89/Sr microcuries per mg Sr. The estimated radioactivity located in the bone surface was 4200, 3000 and 2400 cpm/mg bone when measured 2 days after the administration of /sup 89/Sr, and was lost with an estimated biological half-life of 27, 25, and 23 days, respectively. Bone marrow suppression was assessed by quantitation of the depletion of macrophage-colony forming cells (M-CFC) grown in vitro. The decline in M-CFC closely paralleled the level of radioactivity in the bone. These effects were clearly reflected by the depletion of monocytes in the blood, which were reduced to 14%, 14%, and 21% of control levels corresponding to SA's of 6 Curies/mg, 100 microcuries/mg and 20 microcuries/mg when counted on day 10. By day 30 the respective monocyte levels were 15%, 31%, and 77%. Furthermore, the induction of prostaglandin E producing suppressor macrophages (marophages) by Corynebacterium parvum administration was found to vary inversely with the effects of radioactivity in the bone, with initial impairment followed by quantitative recorvery. Resident-type macrophages in impairment cavity appear to be unaffected by /sup 89/Sr-treament. These data suggest that the monocytes and suppressor macrophages are dependent on radiosensitive marrow cells.

• Research Organization: Medical Coll. of Pennsylvania, Philadelphia (USA). Dept. of Microbiology and Immunology
• OSTI ID: 5802744
• Report Number(s): AD-A-165530/7/XAB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANEMIAS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY
BODY FLUIDS
BONE MARROW
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
MONOCYTES
NUCLEI
ORGANS
PHAGOCYTES
PROSTAGLANDINS
RADIATION EFFECTS
RADIOISOTOPES
RADIOSENSITIVITY
RODENTS
SOMATIC CELLS
SPLEEN
STRONTIUM 89
STRONTIUM ISOTOPES
SYMPTOMS
TISSUES
VERTEBRATES