Specific binding sites for growth hormone in cultured mouse thymic epithelial cells

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90147-4· OSTI ID:5802628

Ban, E; Haour, F ^[1]; Gagnerault, M; Dardenne, M; Postel-Vinay, M ^[2]; Jammes, H ^[3]

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1. Institut Pasteur, Paris (France)
2. Hopital Necker, Paris (France)
3. Endocrinologie Molecuaire, INRA, Jouy-en-Josas (France)

Growth hormones bound specifically to murine thymic epithelial cells, which represent the major component of thymic micro-environment and can be modulated by pituitary hormones. The Kds found with human growth hormone and bovine growth hormone were 0.14 and 0.27 nM with a Bmax 0.56 and 0.35 fmol/10{sup 6} cells respectively. Competition experiment analysis showed ED{sub 50} of 0.24 nM for hGH, 0.46 nM for rGH, 0.71 nM for bGH, 11.8 nM for hPRL and 11.2 nM for oPRL. No specific binding of ({sup 125}I)-oPRL was observed under the same conditions. Both hPRL and bGH showed a negative regulatory effect on the number of the hGH binding sites when incubated with the culture for three days. The presence of GH receptors on thymic epithelial cells provides biochemical evidence for the effect of GH on thymic function.

• OSTI ID: 5802628
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 48:22; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BODY
CATTLE
CELL CULTURES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
FUNCTIONS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LYMPHATIC SYSTEM
MAMMALS
MAN
MEMBRANE PROTEINS
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
RUMINANTS
SOMATIC CELLS
STH
THYMUS
THYMUS CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES