Mutagenic and lethal effects of (5-/sup 125/I)lodo-2'-deoxyuridine incorporated into DNA of mammalian cells, and their RBEs

Journal Article · Tue Dec 01 00:00:00 EST 1981 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575635· OSTI ID:5795013

Miyazaki, N; Fujiwara, Y

Decay of /sup 125/I unifilarly incorporated as 5-iodo-2'-deoxyuridine (IdUrd) into DNA of V79 Chinese hamster cells was approximately an order of magnitude more effective in inducing both 6-thioguanine-resistant mutation and cell inactivation than external X rays under equivalent conditions. RBEs of mutation and killing induced by /sup 125/I decays, compared with 170-kVp X rays of low LET, were approx. = 11 for mutation (ratio of the induction rate in frequency/rad = 11.3 X 10/sup -7/ (/sup 125/I)/100 X 10/sup -7/ (X rays at -79/sup o/C)) and approx. = 10 for cell inactivation (D/sub 0/ ratio = 505 rad (X rays at -79/sup o/C)/52 rad (/sup 125/I)). These RBE values may well exceed the reported maximum values for high-LET radiation in the LET range of 80-110 keV/..mu..m, suggesting that the Auger effect is different from the high-LET radiation effect alone. Thus these biological consequences arise not only from radiation effects of Auger electrons on the immediate vicinity in DNA, but also from the nonionogenic effect through charge transfer processes. In addition, higher inductions of mutation and killing by external X rays in unifilarly IdUrd-substituted cells than in ordinal cells were observed, suggesting a possible involvement of X-ray-induced Auger phenomenon in iodine in DNA.

• Research Organization: Kobe Univ. School of Medicine
• OSTI ID: 5795013
• Journal Information: Radiat. Res.; (United States), Vol. 88:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
MUTAGENESIS
RBE
IODINE 125
BIOLOGICAL RADIATION EFFECTS
X RADIATION
COMPARATIVE EVALUATIONS
DNA
MUTAGENS
MUTATIONS
RADIATION DOSES
URIDINE
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
DAYS LIVING RADIOISOTOPES
DOSES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
IONIZING RADIATIONS
ISOTOPES
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RIBOSIDES
URACILS
560114* - Radiation Effects on Biochemicals- In Animals- (-1987)