Ethanol fermentation of crude acid hydrolyzate of cellulose using high-level yeast inocula
High-level yeast inocula was investigated as a means of overcoming the toxicity problem in ethanol fermentation of acid hydrolyzate of wood cellulose. When the inoculum level exceeded 10/sup 8/ initial cells/mL, 50% of the yeast cells survived the initial cell death period during which furfural and HMF were depleted. The fermentation thus proceeded to completion by virtue of cell regrowth. The specific ethanol productivity in batch fermentation on the basis of viable cells was comparable to that of pure glucose fermentation. Continuous fermentation with cell recycle was superior to batch fermentation in that there was no overall cell decline and the ethanol yield was substantially higher. The maximum ethanol productivity in continuous fermentation was 4.9 g/L h and it occurred at a dilution rate of 0.24 hr/sup -1/.
- Research Organization:
- Auburn Univ., AL
- OSTI ID:
- 5793312
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:3; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
ACID HYDROLYSIS
ALCOHOLS
BIOCONVERSION
BIOSYNTHESIS
CARBOHYDRATES
CELL RECYCLE
CELLULOSE
CHEMICAL REACTIONS
CONTINUOUS CULTURE
DATA
DECOMPOSITION
ETHANOL
EXPERIMENTAL DATA
FERMENTATION
FUNGI
HYDROLYSIS
HYDROXY COMPOUNDS
INFORMATION
LYSIS
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PRODUCTIVITY
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
TOXICITY
YEASTS