Mutagenic effects of sodium azide in rice

Journal Article · Mon Sep 01 04:00:00 EDT 1980 · Crop Sci.; (United States)

DOI:https://doi.org/10.2135/cropsci1980.0011183X002000050030x· OSTI ID:5791931

Awan, M A; Konzak, C F; Rutger, J N; Nilan, R A

Seeds of rice cultivar M5 were presoaked in distilled water and treated for 2 or 3 hours with 0, 0.12, 0.50, 0.75, 1.0, 1.25, 1.50, and 1.75 mM sodium azide solutions prepared in 0.1 M phosphate buffer (pH 3). Criteria used to assess the biological effects of azide on rice were germination, seedling height, and seed sterility in the M/sub 1/ generation, and chlorophyll-deficient seedlings and viable mutations in the M/sub 2/ generation. In general, an increase in azide concentration, along with an increase in the post-treatment redrying period, resulted in a decrease in M/sub 1/ germination and seedling height. Azide treatment also induced sterility. The same treatment induced chlorophyll mutations in 98.5% of the M/sub 1/ panicle progenies and in 14% of the M/sub 2/ seedlings. The highest frequency of viable mutations scored in the adult plant stage was 4.64% on an M/sub 2/ plant basis. All azide concentrations were mutagenic.

• Research Organization: Washington State Univ., Pullman
• OSTI ID: 5791931
• Journal Information: Crop Sci.; (United States), Journal Name: Crop Sci.; (United States) Vol. 20; ISSN CRPSA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
553000 -- Agriculture & Food Technology
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
AZIDES
BIOLOGICAL EFFECTS
CEREALS
GENETIC EFFECTS
GERMINATION
GRAMINEAE
GRASS
MUTAGENESIS
NITROGEN COMPOUNDS
PLANTS
RICE
SEEDLINGS
SEEDS
SODIUM COMPOUNDS
VIABILITY