Radioimmune and radioreceptor study of the pharmacokinetics of the enkephalin analog dalargin in man
Journal Article
·
· Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
The aim of this investigation was to study the pharmacokinetics of dalargin in many by combined use of radioimmune and radioreceptor methods. To study the pharmacokinetics of dalargin, an antiserum not cross-reacting with enkephalins or with various fragments and analogs of dalargin was used. Dalargin was iodinated with the aid of chloramine, and labeled peptide was separated from free iodine by gel-filtration with the use of Bio-Gel P-2. Radioreceptor analysis was carried out with a preparation of lyophilized rat brain membranes containing opiate receptors. To 300 microliters of membrane suspension were added 200 microliters of blood plasma, diluted, 50 microliters of /sup 3/H-D-Ala/sup 2/, and D-Leu/sup 5/-enkephalin.
- OSTI ID:
- 5781630
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 101:6; ISSN BEXBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL REACTIONS
COUNTING TECHNIQUES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENDORPHINS
ENKEPHALINS
HALOGENATION
IMMUNOASSAY
IMMUNOLOGY
INJECTION
INTAKE
INTRAVENOUS INJECTION
IODINATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LABELLING
LYOPHILIZATION
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
NARCOTICS
NERVE CELLS
NEUROREGULATORS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PEPTIDES
PHARMACOLOGY
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
SCINTILLATION COUNTING
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULCERS
VERTEBRATES
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL REACTIONS
COUNTING TECHNIQUES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENDORPHINS
ENKEPHALINS
HALOGENATION
IMMUNOASSAY
IMMUNOLOGY
INJECTION
INTAKE
INTRAVENOUS INJECTION
IODINATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LABELLING
LYOPHILIZATION
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
NARCOTICS
NERVE CELLS
NEUROREGULATORS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PEPTIDES
PHARMACOLOGY
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
SCINTILLATION COUNTING
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULCERS
VERTEBRATES