Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Regulation of low density lipoprotein receptor function in a human hepatoma cell line

Journal Article · · Hepatology (N.Y.); (United States)
; ;
Low density lipoprotein (LDL) processing was investigated in a human hepatoma-derived cell line, Hep G2. Hep G2 cells bound, internalized and degraded LDL via a saturable, high affinity pathway similar to that present in other mammalian cells. Although 80% of the uptake and degradation of /sup 125/I-LDL was inhibited by 40-fold excess native LDL, the same concentration of methylated LDL, which cannot bind to LDL receptors, had virtually no effect on processing. When added at low concentrations, the lysosomotropic agent, chloroquine, inhibited degradation without affecting the rate of lipoprotein internalization. Receptor activity was decreased 60% by preincubation of the cells in medium containing a source of cholesterol (LDL or unesterified cholesterol) and increased 1.7-fold by preincubation with compactin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase. The Hep G2 cell line may prove a useful system both for the further study of hepatic lipoprotein metabolism and for the evaluation of new antihypercholesterolemic agents.
Research Organization:
Massachusetts Inst. of Tech., Cambridge
OSTI ID:
5778988
Journal Information:
Hepatology (N.Y.); (United States), Journal Name: Hepatology (N.Y.); (United States) Vol. 4:5; ISSN HEPAD
Country of Publication:
United States
Language:
English

Similar Records

Stimulation of LDL receptor activity in Hep-G2 cells by a serum factor(s)
Journal Article · Sun May 01 00:00:00 EDT 1988 · J. Cell. Physiol.; (United States) · OSTI ID:6859875
Effect of doxazosin on cholesterol synthesis in cell culture
Journal Article · Sat Dec 31 23:00:00 EST 1988 · J. Cardiovasc. Pharmacol.; (United States) · OSTI ID:5723176
Low-density lipoprotein as a potential vehicle for chemotherapeutic agents and radionucleotides in the management of gynecologic neoplasms
Journal Article · Tue Apr 14 23:00:00 EST 1981 · Am. J. Obstet. Gynecol.; (United States) · OSTI ID:5584337

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HEPATOMAS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIPIDS
LIPOPROTEINS
MAMMALS
MAN
METABOLISM
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
TUMOR CELLS
UPTAKE
VERTEBRATES