Gonadotropin-releasing hormone (Gn-RH) in striped mullet (Mugil cephalus), milkfish (Chanos chanos), and rainbow trout (Salmo gairdneri): comparison with salmon Gn-RH
Immunoreactive gonadotropin-releasing hormone (Gn-RH) was extracted from brains of striped mullet, milkfish, rainbow trout, and chum salmon with acetone/HCl and petroleum ether. High pressure liquid chromatography and cross-reactivity studies show mullet, milkfish, and trout brains to contain a peptide chromatographically and immunologically identical to synthetic salmon Gn-RH, while the mammalian form of Gn-RH is detectable in none of these fishes. Gn-RH is present in immature 7-month-old and 4-year-old milkfish. A second immunoreactive peptide is separable by HPLC in all the fish studied. This early-eluting form of Gn-RH is unlikely to be a precursor; its cross-reactivity with antisera R-42 and number185 suggests that any modification is in the C-terminal region. Several possible roles for this peptide are advanced.
- Research Organization:
- Univ. of Victoria, British Columbia, Canada
- OSTI ID:
- 5778927
- Journal Information:
- Gen. Comp. Endocrinol.; (United States), Vol. 55:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IODINE ISOTOPES
RADIOIMMUNOASSAY
LIBERINS
FISHES
GONADOTROPINS
PEPTIDES
SALMON
TROUT
ANADROMOUS FISHES
ANIMALS
AQUATIC ORGANISMS
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOASSAY
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics