Applications of isotope differentiation for metabolic studies with di-(2-ethylhexyl) phthalate

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Environ. Sci. Health, Part B; (United States)

DOI:https://doi.org/10.1080/03601238209372351· OSTI ID:5778525

Albro, P W; Hass, J R; Peck, C C; Jordan, S T; Corbett, J T; Schroeder, J

The pervasiveness of the plasticizer di-(2-ethylhexyl) phthalate (DEHP) in the environment and especially in the laboratory results in a background that may cause severe interference with analytical studies. Animal-to-animal variability in the distribution of DEHP metabolites in excreta normally makes it necessary to use large groups of animals when different treatments are compared. Finally, radioactive tracers are usually considered undesirable for metabolic studies involving human subjects. All of these problems can be overcome through the use of multiple isotopic labels, especially /sup 12/C//sup 13/C//sup 14/C. Examples are given involving rats and monkeys, and applicability to humans is discussed. The principles involved are not limited to any particular class of test compounds. In rats, the competing pathways for metabolism of phthalate esters produce a different distribution of metabolites from a small intravenous dose of DEHP than from a large oral dose.

• Research Organization: National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina
• OSTI ID: 5778525
• Journal Information: J. Environ. Sci. Health, Part B; (United States), Journal Name: J. Environ. Sci. Health, Part B; (United States) Vol. 17:6; ISSN JPFCD
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL PATHWAYS
CARBON 12
CARBON 13
CARBON 14 COMPOUNDS
CARBON ISOTOPES
ESTERS
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
METABOLISM
NUCLEI
ORGANIC COMPOUNDS
PHTHALIC ACID ESTERS
STABLE ISOTOPES
TRACER TECHNIQUES