Effect of hypothermia on cell kinetics and response to hyperthermia and x rays

Journal Article · Fri Feb 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576395· OSTI ID:5777654

van Rijn, J; van den Berg, J; Kipp, J B.A.; Schamhart, D H.J.; van Wijk, R

Hyperthermia is a potent radio enhancer. Studies using hypothermia in combination with irradiation have given confusing results due to lack of uniformity in experimental design. This report shows that hypothermia might have potential significance in the treatment of malignant cells with both thermo- and radiotherapy. Reuber H35 hepatoma cells, clone KRC-7 were used to study the effect of hypothermia on cell kinetics and subsequent response to hyperthermia and/or X rays. Cells were incubated at 8.5/sup 0/C or between 25 and 37/sup 0/C for 24 hr prior to hyperthermia or irradiation. Hypothermia caused sensitization to both hyperthermia and X rays. In contrast to the effect of hypothermia on either hyperthermia or X rays alone, thermal radiosensitization was decreased in hypothermically pretreated cells (24 hr at 25/sup 0/C) compared to control cells (37/sup 0/C). The expression of thermotolerance and the rate of development at 37/sup 0/C after an initial heating at 42.5/sup 0/C were not influenced after preincubation at 25/sup 0/C for 24 hr. The expression of thermotolerance for heat or heat plus X rays during incubation at 41/sup 0/C occurred in a significantly smaller number of cells after 24 hr preincubation at 25/sup 0/C. The enhanced thermo- and radiosensitivity in hypothermically treated cells disappeared in approximately 6 hr after return to 37/sup 0/C.

• Research Organization: Free Univ., Amsterdam, Netherlands
• OSTI ID: 5777654
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY TEMPERATURE
CELL CYCLE
CELL KILLING
CELL PROLIFERATION
DATA
DISEASES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HEPATOMAS
HYPERTHERMIA
HYPOTHERMIA
INFORMATION
IONIZING RADIATIONS
KINETICS
NEOPLASMS
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
TEMPERATURE EFFECTS
TUMOR CELLS
X RADIATION