Investigation of the biotransformation of promutagens by the Ames test. Communication II. Degree of mutagenic action of nitrosomorpholine, diethylnitrosamine, and cyclophosphane, using various subfractions of a rat liver homogenate

Journal Article · Sun Jun 01 00:00:00 EDT 1986 · Sov. Genet. (Engl. Transl.); (United States)

OSTI ID:5777004

Loknitskaya, N N; Fonshtein, L M

The work presents data on the participation of the components of the microsomal and cytosol subfractions of the S-9 fraction of rat liver homogenates in processes leading to the formation of active metabolites of promutagens nitrosomorpholine (NM), diethylnitrosamine (DENA), and cyclophosphane (CP) and their detoxification as a result of the reaction with glutathione (G-SH), added to the system. It was established that the metabolic activation of NM is associated only with the microsome subfractions, whereas the reactions of the first phase of CP and DENA metabolism occur when both microsomal and cytosol subfractions are added. A decrease in the effect of all the promutagens studied under the influence of G-SH was added when the microsomal and cytosol subfractions of the fraction S-9 were introduced into the activating mixture. Various dependences of the level of metabolic activation and degree of reduction of the mutagenic effect from the addition of G-SH on the protein content in the microsomal and cytosol subfractions were detected for the investigated promutagens.

• Research Organization: Scientific-Research Institute for the Biological Testing of Chemical Compounds, Moscow, USSR
• OSTI ID: 5777004
• Journal Information: Sov. Genet. (Engl. Transl.); (United States), Vol. 21:12; Other Information: Translated from Genetika; 21: No. 12, 1932-1935(Dec 1985)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
LIVER CELLS
MUTAGENESIS
MICROSOMES
MORPHOLINES
GENETIC EFFECTS
MUTAGEN SCREENING
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
CELL CULTURES
CYTOLOGY
DETOXIFICATION
ENZYMES
METABOLIC ACTIVATION
METABOLISM
MUTATIONS
PROTEINS
RATS
REVERTANTS
SALMONELLA TYPHIMURIUM
AMINES
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGY
CELL CONSTITUENTS
ETHERS
HETEROCYCLIC COMPOUNDS
MAMMALS
MICROORGANISMS
MUTANTS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
RODENTS
SALMONELLA
SCREENING
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550500 - Metabolism
550400 - Genetics