Energy-linked sulfate uptake by corn mitochondria via the phosphate transporter

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.63.4.635· OSTI ID:5774877

Abou-Khalil, S; Hanson, J B

Corn shoot mitochondria possess an energy-linked transport system for sulfate uptake as demonstrated by osmotic swelling and (/sup 35/S)SO/sub 4//sup 2 -/ accumulation. Maximum uptake is secured in the presence of Mg/sup 2 +/ and oligomycin with sucrose for osmotic support. Neither phosphate nor dicarboxylate anions are required. When added simultaneously, millimolar concentrations of phosphate block (/sup 35/S)SO/sub 4//sup 2 -/ uptake after the initial minute. Mersalyl, N-ethylmaleimide, and 2,4-dinitrophenol are strong inhibitors of sulfate uptake; n-butylmalonate is a weak inhibitor. These inhibitors act in the same fashion on phosphate uptake. It is concluded that sulfate uptake in the absence of phosphate is by the phosphate transporter.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 5774877
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 63; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CEREALS
DAYS LIVING RADIOISOTOPES
DINITROPHENOL
ELEMENTS
EVEN-ODD NUCLEI
GRAMINEAE
GRASS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNESIUM
MAIZE
MEMBRANE TRANSPORT
METALS
MITOCHONDRIA
NITRO COMPOUNDS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXYGEN COMPOUNDS
PHENOLS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PLANTS
RADIOISOTOPES
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
UPTAKE