Arsenic species separation by IELC-ICP/OES: Arsenocholine behavior

Journal Article · Fri Jan 01 00:00:00 EST 1993 · Journal of Liquid Chromatography; (United States)

DOI:https://doi.org/10.1080/10826079308019706· OSTI ID:5771666

Rubio, R; Peralta, I; Alberti, J; Rauret, G ^[1]

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1. Univ. de Barcelona (Spain)

In the literature an increasing interest is observed in developing methods to determine arsenobetaine, arsenocholine and related compounds in sea food and in reference materials. The separation conditions and quantification of As(III), As(V), monomethylarsenate (MMA), dimethylarsinate (DMA), arsenobetaine (AsBet) and arsenocholine (AsChol) are studied by Liquid Chromatography (LC) coupled directly to an Inductively Coupled Plasma Optical Emission Spectroscopy (ICP/OES) system. The separation conditions are optimized to improve the resolution of the six arsenic species. Arsenocholine shows a particular pattern of behavior when phosphate is used as eluent: two peaks are observed in the chromatogram, thus a systematic study assaying different pH and concentration of phosphate is carried out to improve resolution and analysis time when the six arsenic compounds are analyzed in a mixture. Boric acid as mobile phase avoids the splitting of the arsenocholine peak and leads to a good separation of the six arsenic compounds. Detection limits are established for the six arsenic species.

• OSTI ID: 5771666
• Journal Information: Journal of Liquid Chromatography; (United States), Vol. 16:16; ISSN 0148-3919
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ARSENATES
SEPARATION PROCESSES
ARSENIC
SEAFOOD
CONTAMINATION
BIOLOGICAL ACCUMULATION
CHOLINE
EMISSION SPECTROSCOPY
LIQUID COLUMN CHROMATOGRAPHY
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ARSENIC COMPOUNDS
CHROMATOGRAPHY
DRUGS
ELEMENTS
FISH PRODUCTS
FOOD
HYDROXY COMPOUNDS
LIPOTROPIC FACTORS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
QUATERNARY COMPOUNDS
SEMIMETALS
SPECTROSCOPY
560300* - Chemicals Metabolism & Toxicology
400105 - Separation Procedures