Bioaccumulation of nickel by intercalation into polycrystalline hydrogen uranyl phosphate deposited via an enzymatic mechanism
- Univ. of Birmingham (United Kingdom)
A Citrobacter sp. accumulates uranyl ion (UO{sub 2}{sup 2+}) as crystalline HUO{sub 2}PO{sub 4}{center_dot}4H{sub 2}O (HUP), using enzymatically generated inorganic phosphate. Ni was not removed by this mechanism, but cells already loaded with HUP removed Ni{sup 2+} by intercalative ion-exchange, forming Ni(UO{sub 2}PO{sub 4}){sub 2}{center_dot}7H{sub 2}O, as concluded by x-ray diffraction (XRD) and proton induced x-ray emission (PIXE) analyses. The loaded biomass became saturated with Ni rapidly, with a molar ratio of Ni:U in the cellbound deposit of approx. 1:6; Ni penetration was probably surface-localized. Cochallenge of the cells with Ni{sup 2+} and UO{sub 2}{sup 2+}, and glycerol 2-phosphate (phosphate donor for phosphate release and metal bioprecipitation) gave sustained removal of both metals in a flow through bioreactor, with more extensively accumulated Ni. We propose `Microbially Enhanced Chemisorption of Heavy Metals` (MECHM) to describe this hybrid mechanism of metal bioaccumulation via intercalation into preformed, biogenic crystals, and note also that MECHM can promote the removal of the transuranic radionuclide neptunium, which is difficult to achieve by conventional methods. 42 refs., 1 fig., 1 tab.
- OSTI ID:
- 577022
- Journal Information:
- Bio/Technology, Journal Name: Bio/Technology Journal Issue: 5 Vol. 14; ISSN 0733-222X; ISSN BTCHDA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
29 ENERGY PLANNING, POLICY, AND ECONOMY
32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL ACCUMULATION
BIOLOGICAL RECOVERY
CALCINATION
CHEMISORPTION
CLATHRATES
DECONTAMINATION
ENZYME ACTIVITY
ION EXCHANGE
NEPTUNIUM
NICKEL
PHOSPHATASES
REMEDIAL ACTION
UPTAKE
URANYL PHOSPHATES
WASTE PROCESSING
WASTE WATER
X-RAY DIFFRACTION
X-RAY EMISSION ANALYSIS
32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL ACCUMULATION
BIOLOGICAL RECOVERY
CALCINATION
CHEMISORPTION
CLATHRATES
DECONTAMINATION
ENZYME ACTIVITY
ION EXCHANGE
NEPTUNIUM
NICKEL
PHOSPHATASES
REMEDIAL ACTION
UPTAKE
URANYL PHOSPHATES
WASTE PROCESSING
WASTE WATER
X-RAY DIFFRACTION
X-RAY EMISSION ANALYSIS