Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system

Journal Article · Tue Oct 15 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0540· OSTI ID:577008

Shen, Zhiyuan; Pardington-Purtymun, P E; Comeaux, J C ^[1]

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1. Los Alamos National Labs., NM (United States); and others

The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the bait in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52`s self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I. 22 refs., 3 figs.

• OSTI ID: 577008
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 37; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ANIMAL CELLS
CELL CYCLE
DNA REPAIR
DNA SEQUENCING
DNA-CLONING
ENZYMES
FLUORESCENCE
GENE RECOMBINATION
GENE REGULATION
GENETIC MAPPING
HUMAN CHROMOSOME 16
HYBRIDIZATION
IN-SITU HYBRIDIZATION
MEIOSIS
MITOSIS
PROTEINS
STRAND BREAKS
STRUCTURE-ACTIVITY RELATIONSHIPS
YEASTS