Structural changes in plasma membranes prepared from irradiated Chinese hamster V79 cells as revealed by Raman spectroscopy

Journal Article · Mon Apr 01 00:00:00 EST 1991 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3578167· OSTI ID:5768778

Verma, S P; Sonwalkar, N ^[1]

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1. Tufts Univ. School of Medicine, Boston, MA (USA)

The effect of gamma irradiation on the integrity of plasma membranes isolated from Chinese hamster V79 cells was investigated by Raman spectroscopy. Plasma membranes of control V79 cells show transitions between {minus}10 and 5{degree}C (low-temperature transition), 10 and 22{degree}C (middle-temperature transition), and 32 and 40{degree}C (high-temperature transition). Irradiation (5 Gy) alters these transitions markedly. First, the low-temperature transition shifts to higher temperature (onset and completion temperatures 4 and 14{degree}C). Second, the middle-temperature transition shifts up to the range of about 20-32{degree}C, but the width remains unchanged. Third, the higher temperature transition broadens markedly and shifts to the range of about 15-40{degree}C. Protein secondary structure as determined by least-squares analysis of the amide I bands shows 36% total helix, 55% total beta-strand, and 9% turn plus undefined for control plasma membrane proteins. Plasma membrane proteins of irradiated V79 cells show an increase in total helix (40 and 45% at 5 and 10 Gy, respectively) and a decrease in the total beta-strand (48 and 44% at 5 and 10 Gy, respectively) structures. The qualitative analysis of the Raman features of plasma membranes and model compounds in the 1600 cm-1 region, assigned to tyrosine groups, revealed that irradiation alters the microenvironment of these groups. We conclude that the radiation dose used in the survival range of Chinese hamster V79 cells can cause damage to plasma membrane proteins without detectable lipid peroxidation, and that the altered proteins react differently with lipids, yielding a shift in the thermal transition properties.

• OSTI ID: 5768778
• Journal Information: Radiation Research; (USA), Vol. 126:1; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL MEMBRANES
BIOLOGICAL RADIATION EFFECTS
ANIMAL CELLS
GAMMA RADIATION
HAMSTERS
LUNGS
MEMBRANE PROTEINS
RAMAN SPECTRA
TEMPERATURE DEPENDENCE
ANIMALS
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
RODENTS
SPECTRA
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture