Coordinate turnover of nuclear and cytoplasmic histone messenger RNA following inhibition of DNA replication of HeLa S3 cells

Journal Article · Tue May 19 04:00:00 EDT 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00384a040· OSTI ID:5768463

Bandyopadhyay, R; Stein, G; Stein, J

The authors have examined the metabolism of human H4 histone mRNA in the nucleus and cytoplasm of HeLa S3 cells following inhibition of DNA synthesis to address the extent to which histone mRNA stability in these cellular compartments is coupled to DNA replication. The nuclear and cytoplasmic levels of histone mRNAs encoded by the pF0108A human H4 histone gene were determined by S1 nuclease analysis using a /sup 32/P-labeled probe that could distinguish pF0108A transcripts from those of other members of the H4 histone multigene family. Hydroxyurea treatment resulted within 15 min in a 75% reduction in the level of histone H4 mRNA in the nucleus, which corresponds to the 85% decrease observed for H4 histone mRNA in the cytoplasm. The kinetics of nuclear and cytoplasmic H4 mRNA turnover following hydroxyurea treatment were also similar. Northern blot analysis using a /sup 32/P-labeled mitochondrial cytochrome b probe indicated that the association of cytoplasmic RNA with the nuclear fraction was less than 0.5%. Treatment of cells with a protein synthesis inhibitor resulted in a 1.3-fold increase in nuclear H4 histone mRNA levels and a 1.5-fold increase of H4 mRNA in the cytoplasm after 45 min. Together, these results indicate that nuclear and cytoplasmic H4 histone mRNAs respond similarly to metabolic perturbations that influence message stability and that mechanisms operative in the turnover of histone mRNAs in the nucleus and cytoplasm may be similar.

• Research Organization: Univ. of Florida College of Medicine, Gainesville
• OSTI ID: 5768463
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:10; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
ATP
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DNA REPLICATION
ENZYMES
ESTERASES
HELA CELLS
HETEROCYCLIC COMPOUNDS
HISTONES
HYDROLASES
HYDROXY COMPOUNDS
HYDROXYUREA
INHIBITION
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MESSENGER-RNA
METABOLISM
NUCLEASES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
REACTION KINETICS
RIBOSIDES
RNA
SCINTILLATION COUNTING
THYMIDINE
TRITIUM COMPOUNDS