Incorporation of deuterium-labeled trans- and cis-13-octadecenoic acids in human plasma lipids
The absorption and distribution of deuterated trans- and cis-13-octadecenoic acid (13t-18:1 and 13c-18:1) in plasma lipids were compared to deuterated cis-9-octadecenoic acid (9c-18:1) in two young adult male subjects. A mixture of triglycerides was fed in a multiple-labeled experiment where each triglyceride contained a fatty acid labeled with a different number of deuterium atoms. Analysis of human plasma lipids by mass spectroscopy allowed the distribution of the two 13-octadecenoic acid isomers to be directly compared to cis-9-octadecenoic acid. Plasma lipids selectively excluded both the 13t-18:1 and 13c-18:1 isomers relative to 9c-18:1 in all neutral and phospholipid fractions. Discrimination against incorporation of the 13t-18:1 isomer into plasma cholesteryl ester and 2-acyl phosphatidylcholine was nearly absolute. The 1-acyl phosphatidylcholine fraction exhibited a large positive selectivity for the 13t-18:1 isomer. Differences in the relative distribution of the trans and cis 13-18:1 isomers vs. 9c-18:1 in the various lipoprotein lipid classes were found. Analysis of the chylomicron triglyceride component of the plasma lipids indicated all three fatty acids were equally well absorbed.
- Research Organization:
- Northern Regional Research Center, U.S. Department of Agriculture, Peoria, IL
- OSTI ID:
- 5764902
- Journal Information:
- J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 24:1; ISSN JLPRA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CARBOXYLIC ACIDS
CHOLESTEROL
DEUTERIUM COMPOUNDS
ESTERS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOMERS
ISOTOPE APPLICATIONS
LIPIDS
LIPOPROTEINS
MATERIALS
METABOLISM
MONOCARBOXYLIC ACIDS
OCTADECANOIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PROTEINS
STEROIDS
STEROLS
TRACER TECHNIQUES
TRIGLYCERIDES
UPTAKE