Isolation of a Pseudomonas stutzeri strain that degrades o-xylene

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5764761

Baggi, G; Barbieri, P; Galli, E; Tollari, S

A Pseudomonas stutzeri strain capable of growing on o-xylene was isolated from enrichment cultures. The organism grew on 2,3- and 3,4-dimethylphenol but not on 2-methylbenzyl alcohol, o-tolualdehyde, or o-toluate. P. stutzeri was not able to utilize m-xylene, p-xylene, or 1,2,4-trimethylbenzene, but growth was observed in the presence of the corresponding alcohols and acids. From the Pseudomonas cultures supplied with o-xylene, 2,3-dimethylphenol was isolated and identified. When resting P. stutzeri cells were incubated with 2,3-dimethylphenol, the reaction mixture turned greenish yellow and showed spectral properties identical to those of the 3,4-dimethylcatechol meta ring fission product. Catechol 2,3-oxygenase was induced by growth on o-xylene or on 2,3- or 3,4-dimethylphenol. The suggested hypothesis is that the first metabolic steps of growth on o-xylene involve the direct oxygenation of the aromatic nucleus, followed by meta pathway reactions.

• Research Organization: Universita degli Studi di Milano, Italy
• OSTI ID: 5764761
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:9; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
GROWTH
HYDROCARBONS
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PSEUDOMONAS
XYLENES