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Evaluation of aflatoxin B/sub 1/ mutagenesis: addition of glutathione and glutathione-S-transferase to the Salmonella mutagenicity assay

Journal Article · · Environ. Mutagen.; (United States)
; ;

The effects of glutathione (GSH) and the combination of GSH and glutathione-S-transferase (GST) on aflatoxin B/sub 1/ (AFB/sub 1/) mutagenesis in the Salmonella mutagenicity assay using Salmonella typhimurium strains TA98 and TA100 were tested. Ten concentrations of AFB/sub 1/ (0-1.0 ..mu..g/plate) were added to a liver microsomal homogenate (S9 mix) or to S9 mix containing GSH or S9 mix containing the combination of GSH + GST. One third of the samples were plated directly. Two-thirds were incubated for 30 min at 37/sup 0/C prior to plating, and of those, half included bacteria. The results show that the addition of GSH and GSH + GST affected AFB/sub 1/ mutagenesis by forming the AFB/sub 1/-GSH conjugate and decreasing the availability of AFB/sub 1/-8,9-epoxide. The effect of GST on GSH activity varied with the strain because of the different amounts of S9 mix used. The formation of the AFB/sub 1/-GSH conjugate was verified by using reverse-phase high-performance liquid chromatography for quantitation of AFB/sub 1/ and detection of AFB/sub 1/-GSH.

Research Organization:
Univ. of Arizona, Tucson
OSTI ID:
5764584
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 9:4; ISSN ENMUD
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANTIGENS
BACTERIA
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CHEMISTRY
CHROMATOGRAPHY
DRUGS
ENZYMES
GENETIC EFFECTS
GLUTATHIONE
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
MICROORGANISMS
MUTAGENESIS
NITROGEN TRANSFERASES
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
SALMONELLA
SALMONELLA TYPHIMURIUM
SEPARATION PROCESSES
TOXIC MATERIALS
TOXINS
TRANSFERASES